arrange the DNA fragments in the increasing order of their migration from the wells~ 100bp,500bp,300bp?
hello,
you must keep in mind that during the gel electrophoresis, the smaller fragments more further towards the anode so 100 bp would be higher travelled, 300 bp in the middle and 500bp the slowest.
refer to the link for more info
http://www.biologydiscussion.com/electrophoresis/electrophoresis-with-diagram/57621