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Quick Facts

Medium Of InstructionsMode Of LearningMode Of Delivery
EnglishSelf StudyVideo and Text Based

Courses and Certificate Fees

Fees InformationsCertificate AvailabilityCertificate Providing Authority
INR 1000yesIIT Bombay

The fees for Introduction to Proteomics is : 

Fees typeAmount
Exam feesRs. 1,000

The Syllabus

  • Introduction to amino acids
  • Introduction to Proteins
  • Protein folding & misfolding
  • Introduction to Proteomics
  • Lab session – Protein-protein interaction using label-free biosensors

  • Sample preparation and pre-analytical factors
  • Sample preparation: Pre-analytical factors (contd.)
  • Sample preparation: Protein extraction and quantification
  • One-dimensional electrophoresis
  • Introduction to 2-DE

  • 2-DE: Second dimension, staining & destaining
  • 2-DE: Gel analysis
  • 2-DE Applications
  • 2-DE Applications (contd.) & Challenges
  • Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis

  • 2D-DIGE: Basics
  • 2D-DIGE: Data analysis
  • 2D-DIGE: Applications
  • Systems biology and proteomics – I
  • Systems biology and proteomics - II

  • Fundamentals of mass spectrometry
  • Chromatography technologies
  • Liquid chromatography
  • Mass spectrometry: Ionization sources
  • Mass spectrometry: Mass analyzers

  • MALDI sample preparation and analysis
  • Hybrid mass spectrometry configurations
  • Lab session - Demonstration of Q-TOF MS technology
  • In-gel & in-solution digestion
  • Lab session - Sample preparation: tissue sample preservation technology

  • Introduction to quantitative proteomics
  • SILAC: In vivo labelling
  • iTRAQ: In vitro labelling
  • TMT: In vitro labelling
  • Quantitative proteomics data analysis

  • Proteomics applications
  • Challenges in proteomics
  • OMICS and translational research
  • Lab session – Targeted proteomics using triple quadrupole mass spectrometry
  • Lab session – Targeted proteomics: multiple reaction monitoring

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